What is the estimated size of the protein indicated with the arrow in its native

Question

What is the estimated size of the protein indicated with the arrow in its native state if it is a homotrimer (i.e. at what size would it run on a gel filtration column under non-denaturing conditions)? 70 50 35 28 15 O-195 kDa cannot tell from the given information -130 kDa -65 kDa Fill in the blanks: A protein with many more E and D residues than K and R residues would be charged at physiological pH, and could likely be purified using chromatography negatively, cation exchange negatively, anion exhange positively, cation exchange positively, anion exchange

Explanation / Answer

1. 195kDa

70 x 3 (homotrimer) =approximately, 210

2. negatively, cation exchange

E= Glutamic acid

D= Aspartic acid. These are acidic amino acids with extra COOH group.

K= Lysine, R= Arginine which are basic aminoacids with extra NH2 group.

Here the protein contains more negatively charged acidic amino acids. Thus this protein could likely be purified by cation exchange chromatography. Because cation is positively charged and attract the negatively charged protein. It enhances the movement and separation during chromatography.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.