Do you know how to do with the last question? Thefollowing experiment was perfor

Question

Do you know how to do with the last question?

Thefollowing experiment was performed to determine the number of polypeptide chains in an oligomeric protein Step 1: A sample (660 mg) of an oligomeric protein of MW 132 kDa was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete Step 2: The sample was boiled in 10% HCl for several hours until all of the peptide bonds were hydrolyzed Step 3: Further analysis revealed the presence of 5.5 mg of the DNP derivative shown below. The molecular weight of the DNP derivative shown below is 283.24 g/mol. No other DNP derivatives were det after step 2. tide chains in the Number Calculate the number of oligomeric protein complex. This is an estimate You will need to round your answer polypeptides What is the identity of the DNP derivative shown below? &DNP-Ala aDNP-Ile CH, CH3 CH NH-C-COOH C O O2N O aDNP-Asn

Explanation / Answer

The first answer is correct the oligomeric protein is made up of 4 polypeptide.

The answer to the second question is:

The oligomer was purified to homogeneity at the start of the experiment because no other DNP derivative was detected except one. The same reason can be applied to ascertain the fact that all subunits of the oligomer are identical in sequence (option1) which ensures identical N-terminal aminoacids that is detected as DNP derivative.

NOTE: If you need further clarification as to how the first answer was obtained feel free to comment on the answer. I will be happy to help. Thank you.

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