Two important pieces of genetic code (aside from the promoter, the gene for araC
ID: 143643 • Letter: T
Question
Two important pieces of genetic code (aside from the promoter, the gene for araC, and the replication origin) are located on the pGLO plasmid. What are they and what do they do? 1. 2. Look over the experiment-and based on the samples we will create, diagram the growth or state in each petri dish the expected outcomes (positive or negative growth or positive or negative glow) of the experiment. The agar components are given for you [ | Agar contains Luria Broth (LB) Agar contains Luria Broth (LB), and Ampicillin (AMP) Agar contains Luria Broth (LB), and Ampicillin (AMP) Agar contains Luria Broth (LB), and Ampicillin (AMP), Arabinose (ara)Explanation / Answer
pGLO has reporter genes which are
1: Green fluorescent protein gene (GFP gene): serves as reporter gene and produces visible green light upon exposure to UV
2: Ampicillin resistance gene: serves as reporter gene and impart ampicilin resistance
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