Review Questions 1. Why should a glass slide and a coverslip be held by their ed

Question

Review Questions 1. Why should a glass slide and a coverslip be held by their edges? 2. Explain why a specimen to be viewed under the microscope must be thin_ 3. Describe how a compound microscope should be held and carried. 4. How is the total magnification of a microscope determined? 5. If the eyepiece on a microscope has a magnification of 10X, what is the total magnification with a 15X objective? 6. If the eyepiece on a microscope has a magnification of 15X, what is the total magnification with a 45X objective? A microscope gives a total magnification of 1500X, but the image is too blurry to be useful.What might be the problem with the microscope? 7. An image is located in the lower right hand corner of the field of view. How would you move the slide to center the image? 8.

Explanation / Answer

1. Glass slide and coverslip should be held by their edges due to leaving fingerprints or debris and how easily breakable they are. Leaving fingerprints or debris on either a slide of coverslip will alter the view under the microscope.

2. Specimens viewed with a compound microscope must be very thin, so that light can pass through them. If they were not thin enough for light to pass through them, they would not be visible under a compound microscope.

3. Always carry microscope in both hands. Grasp the arm with one hand and place the other hand under the base for support. Always place the microscope on a level and stable surface.

4. Total magnification (TM) is determined by multiplying the ocular lens by the objective lens. The ocular lens (eyepiece) usually has a magnification of 10x. Light microscopes have 4 objective lenses: scanning power (4x), low power (10x), high- dry power (40x), and oil immersion (100x).

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