BIOL376| Genetics | Practice problem set 1 The following questions may or may no
ID: 142898 • Letter: B
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BIOL376| Genetics | Practice problem set 1 The following questions may or may not appear in class and/or on tests. Which molecule will be translated? a) mRNA b) tRNA c) DNA d) Protein 1) Amino acid 2) Complementary base-pairing allows for a) Spontaneous mutations to occur b) Genes to be expressed as a phenotype c) DNA to serve as its own template for replication d) Replication to be semiconservative Covalent bonds to form between the opposite bases Which of the following are the purine nucleotides in DNA? a) Adenine and thymine b) Cytosine and guanine c) Adenine and cytosine d) Guanine and adenineou e) Thymine and uracil 3) dhage bn 4) True or False?: In a strand of DNA, a hydrogen bond connects the phosphate group of one nuclcotide to 5) A 5) If adenine makes up 23% of the DNA nucleotides in the genome of a plant, what are the percentages of each )Define "antiparale" in the context of a DNA molecule. bipdumearel ppnt ake sugar of the adjacent nucleotide. is an enzyme that synthesizes long chains of polymers or nucleic acids. of the other nucleotides in the genome? Illustrate or define the busic concept of the "central dogma of biology" A good analogy to describe the genetic code, the information contained in our DNA is? qen ) Do the following for the DNA duplex below: Identify the DNA coding strand (non-template strand) and the nucleotides corresponding to the start and stop codons. a) b) Identify the template strand. c) Starting from its 5' end, write the mRNA sequence showing the codons for the amino acids (put spaces or dashes between the codons to identify them). Using three-letter codes, write the amino acid sequence for the polypeptide produced. DNA duplex: 3' -ACTGATAGACTCGTGACAGTATAC...-5n-Explanation / Answer
1) a
2) e
3) d
4) False. It’s a phosphodiester bond.
5) Polymerase
6) Chargaffs rule:
A=T and G=C
Thus, A=T=23% and G=C=67%
7. The DNA strands are antiparallel, when both of the strands run in opposite direction.
8. Central dogma states that DNA makes RNA and RNA makes protein.
11.
a) DNA ligase
b) RNase H and polymerase I
12. Most of the bacterial genomes are small. Thus the whole genome could be replicated very fast because of which single origin of replication would suffice whereas eukaryotic genomes are huge and will consume lot of time if having only one origin of replication.
13. Genome1 : Single stranded DNA and Genome2: Double Stranded DNA
14. a) The reading frame from the second nitrogenous base makes more sense as in that frame ORF(open reading frame) a start codon ATG is present.
b) Non template strand
c) 5’- TCCTTAATCCGAAGT………….TATGC-3’
d) 5’-UCCUUAAUCCGAAGUUUCUUUCCGUCGC-AUG-UUC-UAU-GC-3’
15) Because then a purine always pairs up with pyrimidine (guanine always bond with cytosine or adenine always bonds with thymine). Thus, the newly formed strands formed are not only complementary to their parental template strands but also identical to the other template.
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