5. You obtain a rate of 0.8 AA/min from one of your samples (Sample A). You adde

Question

5. You obtain a rate of 0.8 AA/min from one of your samples (Sample A). You added 10 1 of a 1:10 dilution of Sample A to a total volume of 1.21 ml, what is the corresponding activity (in umol/min/ml) in the original sample? (Hint: you may wish to consult the Calculation Hints page for assistance in calculating this value.) 6. Sample B gave 0.3 AA/min when using a 1:50 dilution in the same assay. What is the corresponding activity (in umol/min/ml) in the original sample? Which sample (A or B) has the higher activity? 7. What does the term activity means to you? (Refer to your Biochemistry Text)

Explanation / Answer

The solution which is mixed then ratio will be 1:10

We have already given 1value which is 1.21ml then original solution will be 1.21×9= 10.89 ml because 1:10 means 1:9 have original solution

6. 1:50 means 1:49 then original solution is 1.21×49=59.28 ml

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