3. You want to determine whether Tral expression is regulated by micro RNAs. a.

Question

3. You want to determine whether Tral expression is regulated by micro RNAs. a. You believe that miR-277 is inhibiting Tral-GFP translation (but not affecting mRNA stability). Which of the following experimental results would you expect to see if this is the case? Select all that apply. A. Tral-GFP mRNA levels are the same regardless of whether the cell expresses miR-277. B. Tral-GFP protein levels are the same regardless of whether the cell expresses miR-277. C. Tral-GFP mRNA levels are lower when the cell expresses miR-277 D. Tral-GFP proteins levels are lower when the cell expresses miR-277.

Explanation / Answer

Since niR-277 is affecting the translation and not the mRNA stability,

The answer according to me are:

A) Tral-GFP mRNA levels are the same regardless of whether the cell expresses miR-227 as it should not affect the mRNA whether it is being expressed of not.

D) Tral-GFP proteins level are lower when the cell expresses mIR-277 as miR 277 should be inhibiting the translation so less protein should be produced if it is expressed.

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