3. You want to determine whether Tral expression is regulated by micro RNAs. You

Question

3. You want to determine whether Tral expression is regulated by micro RNAs.

You believe that miR-277 is inhibiting Tral-GFP translation (but not affecting mRNA stability). Which of the following experimental results would you expect to see if this is the case? Select all that apply.

A. Tral-GFP mRNA levels are the same regardless of whether the cell expresses miR-277.

B. Tral-GFP protein levels are the same regardless of whether the cell expresses miR-277.

C. Tral-GFP mRNA levels are lower when the cell expresses miR-277.

D. Tral-GFP proteins levels are lower when the cell expresses miR-277.

Explanation / Answer

Question 3:

The correct answers are,

A. Tral-GFP mRNA levels are the same regardless of whether the cell expresses miR-277.

D. Tral-GFP proteins levels are lower when the cell expresses miR-277.

Explanation :

According to Central Dogma, RNA is produced using one strand of DNA as a template with the help of an enzyme RNA polymerase through the process of transcription. That mRNA gets translated to protein.

Here we are considering whether Tral expression is regulated by micro RNAs. We believe that miR-277 is inhibiting Tral-GFP translation but not affecting mRNA stability. Therefore Tral-GFP mRNA levels are the same and it does not depend on whether the cell expresses miR-277 or not. As miR-277 inhibits Tral-GFP translation, therefore if the cell expresses miR-277 then Tral-GFP proteins levels would be lower.

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