22)You are drowing a popuiation of celis that why R the othev options wrong, 21,

Question

22)You are drowing a popuiation of celis that why R the othev options wrong, 21, You are growing a population of cells that appear to be resistant to G418, a chemical that is normally toxic to cells. Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase. What would you do to test if this protein confers resistance to the G418 chemical? a. determine if the cells are also making B-galactosidase b. tag the protein with green fluorescent protein to see if it is degraded by G418 c. label a fragment of the gene to see where it is expressed d. clone the gene into a G418-sensitive cell line, then treat these cells with G418 chemical that

Explanation / Answer

D. Is correct.

We have to exactly that presence of neomycin phosphotransferase is exactly the cell to resist the chemical G418 are not.

This can be achieved by cloneing the cells which are sensitive to G418 with this protein then treating it with G418.if the protein neomycin phosphotransferase helps to resist the G418 the cells will grow otherwise the cells will die

If cells grow we can say that the protein is responsible for resistance otherwise not.

A. There is no relation if it is producing beta galatosidase or not

B. Seen neomycin is degarding by the G418 is not seeing G418 degarded by neomycin may be correct.

C. The region it is expressied can not define the function

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