In the laboratory, you decided to investigate the growth of Salmonella typhimuri

Question

In the laboratory, you decided to investigate the growth of Salmonella typhimurium in nutrient broth by using both viable cell counts and optical methods (spectrophotometry) simultaneously. Cultures of Salmonella were incubated in nutrient broth at 37°C and aliquots were serially diluted and spread-plated on nutrient agar plates at indicated times. Simultaneously, the optical density of the culture was measured and indicated in the table below.

Part F - Using the graph generated using absorbance, interpolate the generation time (Gt) for the Salmonella culture. The generation time is:

Part G - Using the graph generated using viable plate counts, interpolate the generation time (Gt) for the Salmonella culture. The generation time is:

Part H- Use the graph generated using absorbance to calculate the generation time (Gt) for the Salmonella culture. The generation time is:

Part I - Use the graph generated using viable plate counts to calculate the generation time (Gt) for the Salmonella culture. The generation time is:

Part J - An explanation as to which data points used for calculating generation time (Gt) will provide you with more confidence to really assess the Gt for the Salmonella culture.

Part K - An explanation as to why are the plots obtained with each method (viable plate count and absorbance) are not superimposed on one another would be:

approximately 60 min/generation approximately 0.38 hr/generation approximately 10 min/generation approximately 1 hr/generation approximately 23 min/generation Time (hrs.)Viable Counts (CFU/mL)OD%Gnm 3.00 x 10 2.90 x 10 3.50 x 10 6.00 x 10 2.40 x 10 1.01 x 10 2.30 x 10 3.10 x 10 3.50 x 10 0 0.5 1.0 1.5 2.0 0.050 0.051 0.065 0.120 0.510 2.000 3.400 4.300 4.400 3.0 3.5 4.0

Explanation / Answer

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2.

J.

K.

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