In the laboratory, you decided to investigate the growth of Salmonella typhimuri

Q: In the laboratory, you decided to investigate the growth of Salmonella typhimuri

Part A. 1.5 hours. Part B. 2 hours. Part C. The log phase. Part D. Although this phase was not clearly detected, it seems like the culture will probably enter the stationary phase between the 3.5 to 4.5 hour. Part E. no, it cannot be detected; and there is no hint from the plot that this culture will enter stationary phase at least up to 4 hours of growth.

ID: 134194 • Letter: I

Question

In the laboratory, you decided to investigate the growth of Salmonella typhimurium in nutrient broth by using both viable cell counts and optical methods (spectrophotometry) simultaneously. Cultures of Salmonella were incubated in nutrient broth at 37°C and aliquots were serially diluted and spread-plated on nutrient agar plates at indicated times. Simultaneously, the optical density of the culture was measured and indicated in the table below.

Plot the data using Excel and label all axes and clarify all scales used. Indicate all growth phases observed for the Salmonella culture. Excel file cells so that you could generate the graph. The two graphs must be generated on the same plot, that is, this will be a 3 axes graph.

Part A - The adaptation phase for the S. typhimurium culture lasted using the absorbance data.

Part B - The exponential phase for the S. typhimurium culture lasted using the absorbance data.

Part C - The name given to this rapid growth phase is:

Part complete

Part D - Did you detect the stationary phase from the plot generated with absorbance data?

Part E - Did you detect the stationary phase from the plot generated with viable plate count data?

1 hour 1.5 hours 0.5 hour 3 hours 2 hours Time (hrs.)Viable Counts (CFU/mL)OD%Gnm 3.00 x 10 2.90 x 10 3.50 x 10 6.00 x 10 2.40 x 10 1.01 x 10 2.30 x 10 3.10 x 10 3.50 x 10 0 0.5 1.0 1.5 2.0 0.050 0.051 0.065 0.120 0.510 2.000 3.400 4.300 4.400 3.0 3.5 4.0

Explanation / Answer

Part A. 1.5 hours.

Part B. 2 hours.

Part C. The log phase.

Part D. Although this phase was not clearly detected, it seems like the culture will probably enter the stationary phase between the 3.5 to 4.5 hour.

Part E. no, it cannot be detected; and there is no hint from the plot that this culture will enter stationary phase at least up to 4 hours of growth.

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In the laboratory, you decided to investigate the growth of Salmonella typhimuri

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In the laboratory, you decided to investigate the growth of Salmonella typhimuri

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