8- A disinfectant should be used in your a)before you begin lab e) after you fin

Question

8- A disinfectant should be used in your a)before you begin lab e) after you finish working d) all of the above 9. A loop or a needle is sterilized present 10-Why is a stain added to a microbe? logy of cells B. To C. D. Make the cells easier to see under them he microscope All of the above 11- The recommended biosafety level(ls) for handling microorganisms represcent A. the potential of the agent to cause disease B. the conditions under which the agent should be safely handled C..Both A and B are correct D. None of the above is applicable 12-The recommended biosafety levels (BSL) for micro-o related to BSL-4? rganisms are described below. Which of the following is A. Agents that B. Agents that C. Agents that can cause d can cause diseases that do not respond to antimicrobial therapy or to vaccines in healthy pose only moderate risk in healthy adults but not likely to spread throughout the though can be readily treatable D. Agents not likely to pose a disease risk to healthy adults 13-Which reagent functions as a decolorizer in the Gram stain? A. Crystal violet B. Iodine C. Copper sulfate D. Alcohol 14-What is the mordant used in the Gram stain A. Iodine B. Crystal violt C. Safranin D. Alcohol 15-In the Schaeffer-Fulton method, malachite green is use to stain A. Vegetative portion of the cell B. Capsule C. Endospores D. The fungi

Explanation / Answer

8. Answer: D) all of the above.

Disinfectants are identified in the form of sntimicrobial agents that would be applied on the surface of the objects that are non living. These would be carrying out destruction of the microorganisms that are unwanted, and are living and would be present on the object’s surface. This may not be necessarily killing all the microorganisms but would be effective against most of the microorganisms. This is most frequently used in hospitals or dental surgeries, of the bathrooms or kitchens for killing infectious microorganisms.

In this case, all the above options are correct. This is because, if there is a spill of a live culture, even if the area is swipe cleaned, it will not be free of the microbes completely. Also, it is important thatbefore every procedure, the are on which one would be working has to be cleaned or disinfected. Also, it is important that after an area is worked at, it should be cleared, so that there is no contamination or any chances of it.

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