I chose e) and I got it marked as wrong. If you were to repeat the Meselson Stah

Question

I chose e) and I got it marked as wrong.

If you were to repeat the Meselson Stahl experiments, but rather than use N15 and N14, you use P32 and P31, how would the resulting DNA bands present in the CsCl gradient change? Remember the initial culturing of bacteria is in the heavy isotope. Select one: a. Only newly synthesized daughter strands would contain heavy P32 b. No strands of DNA would incorporate light P31 as P is not used in the synthesis of nucleic acids. c. The banding pattern would not change and the results would be similar as if you had used N14 and N15 d. You can't tell since Nitrogen and Phosphorus have different atomic masses, therefore, the relative positions of the resulting (H:H, H:L and L:L) DNA bands would differ from what was observed in the Meselson Stahl experiment using heavy and light N. e. A and C are correct. Your answer is incorrect.

Explanation / Answer

The correct answer would be D

the results of the experiment would be completely different from Meselson Stahl experiment.

since P32 is already in use tolabel DNA. It is used in a process called end labelling. Where the ends of DNA are labelled with P32 to promote visualization of small molecules. Option B is incorrect since P is used in DNA synthesis. SInce it is required for the pHosphate bond, which is the Backbone of the DNA.

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