How does the figure demonstrate that EGFR-GFP traffics to the same location as w

Question

How does the figure demonstrate that EGFR-GFP traffics to the same location as wild type EGFR? Fig. 7. Co-localization of GFP and receptor. PAE cells expressing EGFR-wt (clone B11) (c and d) or EGFR-GFP (clone A11) (a and b) were incubated with EGF (200 ng/ml) at 37 degree C for 30 min. Cells were fixed and permcabilized briefly in X-100 buffer and then incubated with anti-EGFR (Ab'225) followed by the secondary antibodies conjugated with Texas Red. Texas Red (6 and d) and GFP fluorescence (a and c) are visualized using conventional epifluorescence microscopy. Bar. 5 pm.

Explanation / Answer

Co-localization of GFP and EGFR:    The internalization kinetics and postendocytic trafficking of EGFR-GFP appear to be essentially similar to that of native EGFR because in porcine aortic endothelial (PAE) cells, the chimera traffics at a rate are similar to that measured for EGFR-wt, which are expressed in these cells and for endogenous receptors in other cells.   The colocalization of EGFR-GFP when compared with EGFR-wt, shows that it behaves essentially unperturbed in its cellular and biochemical properties. Normally EGFR-GFP is activated by EGF as it is regulated by receptor autophosphorylation, i.e. in vivo activity of tyrosine kinase and activation of MAP kinase pathway.

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