How does substrate concentration affect the reaction rate? Is there a linear rel

Question

How does substrate concentration affect the reaction rate? Is there a linear relationship between the increasing substrate concentration and reaction rate? ___ What is the maximum reaction rate? What does it represent? ___ Determine the enzyme volume you will be using, based on your results from previous week. What is that volume? Complete the table for the week 2 procedures, in your report sheet. ___ If a double reciprocal plot (Lineweaver Burk plot) had the following equation: y = 75.46 times + 11.791 Calculate Km and Vmax.

Explanation / Answer

Solution:

The answer to the pre-lab questions are expressed below,

1. The reactant / substrate concentration favours a vital role in reaction mechanism, an increase in substrate concentration favours forward reaction yielding high conversion of products while in lower amount of substrate concentration affects the product formation. In case of reversible reaction results in backward reaction mechanism.

2. The reacion rate represents the speed reaction, in general the reaction rate speed is limited by substrate concentration in simple termed as limiting reagent. In some cases the maximum amount of reaction rate can be achieved by introducing catalysts (Enzyme) an kinetic enhancer favours the increased reaction rate for the high conversion yield of product.

3. Kindly use your previous lab result for computing volume requirement using law of mass.

4. The computation for the equation y = 75.46 x + 11.791 is expressed below,

     Slope (m) = 75.46 , Intercept = 11.791

     Slope = Km/Vmax

     Intercept = 1/Vmax is 11.791 = 1/Vmax

     Vmax = 0.0848

     75.46 = Km/0.0848

     Km = 6.399

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