The biruet reagent is prepared by dissolving 1.5g of copper sulfate and 6g sodiu

Question

The biruet reagent is prepared by dissolving 1.5g of copper sulfate and 6g sodium potassium tartrate in 500 mL oh H2O. Add 300mL of 10% NaOH, and bring the final volume up to 1L. It will keep indefinitely if 1g potassium iodide is added to inhibit the reduction of copper.

d) A sample prepared with 0.50ml of protein sample A and 2.5ml of Biuret reagent gave an absorbance of 0.51. Calculate the protein concentration of the original protein A sample, in g/ml, twice: once with each standard curve. ix. On the calculation sheet (see iii), neatly show all the calculations required to solve for the concentration of the protein sample. Remember to perform this calculation twice: once with each standard curve. Include a title to separate this calculation from others on the page.

The equations from the standard curve graphs are:

y=0.0002x+0.1706

y=0.0011x+0.1706

Explanation / Answer

The biruet reagent is prepared by dissolving 1.5g of copper sulfate and 6g sodiu

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