The binding of the protein TetR to double-stranded DNA involves several ionic bo

Question

The binding of the protein TetR to double-stranded DNA involves several ionic bonds (amongst other non-covalent interactions). Assume that the ethanol concentration inside the cell increases changing the dielectric constant from 80 to 70.

A. What effect would this change have on the strength of the ionic interactions between TetR and DNA? (increase, decrease or have no effect)

B. If the strength of the bonding interactions are changed then quantitatively describe the magnitude of that change (for ex. (some #)x weaker/stronger) due to the change).

C. What effect would you expect this increase in ethanol concentration to have on the number of “non-specific” ionic interactions inside the cell. In other words, would you now have more, or fewer, ionic interactions between molecules relative to the number of interactions that occurred prior to the increase in ethanol concentration? Explain your answer.

Explanation / Answer

There will be decrease in the strength of the ionic interactions between TetR and DNA when dielectric constant change from If the strength of bonding increases then then there will be more interaction between DNA and TetR and if strength of bonding is decreases, then strength is decresed. Increase in ethanol concentration, will increase the non-specific type of increases as ethanol provide the free hydrogen ion.

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