1.)Briefly describe a technque to increase any given quantity of DNA. 2.)A popul
ID: 10693 • Letter: 1
Question
1.)Briefly describe a technque to increase any given quantity of DNA.2.)A population:
a. is the unit of evolution
b. consists of interbreeding members of the same species
c. shares the same gene pool
d. grows at an exponential rate when the birth rate exceeds the death rate at a constant differential, no matter how slight the difference.
e. all of these.
3.)which (is/are) intrinsic isolating mechanisms?
behavioral isolation
mechanical isolation
hybrid infertility
ecological isolation
all of the above
Explanation / Answer
answer 1: Polymerase chain reaction(PCR): is a molecular biology techniques for enzymatically replicating DNA without using a living organism such as E.coil. This technique allows a small amount of DNA molecules to be amplified many times, in an exponential manner and generating thousand to millions of copies of particular DNA sequence. basic components of PCR are 1. DNA TEMPLATE: which contain the region of DNA fragments to be amplified. 2. Two primers: which determines the beginning and ending of the region to be amplified. 3. Taq polymerase: which copies the region to be amplified 4. Nucleotides: from which the DNA –polymerase builds the new DNA 5. Buffers: which provides a suitable chemical environment for the DNA--polymerase The PCR reaction is carried out in a thermal cycler 1 (denaturing) the double stand DNA heated to 94 to 96 degree centigrade for 1 to 2 minutes in order to separate the strands 2 (annealing) temperature is load between 45 to 50 degree for 1 to 2 minutes so the primers can attach themselves to single DNA strands 3 (Extension) the DNA polymerase fills in missing stands it start at the annealed primer and works its way along the DNA stand the time for this step varies from 1 to 5 minutes depending upon length of DNA fragment to be amplified answer 2: e all of these answer 3: mechanical isolation i hope this will help you
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.