Thank you in advance How does marked hemolysis, icterus, or lipemia in a patient

Question

Thank you in advance How does marked hemolysis, icterus, or lipemia in a patient's serum affect results for total protein? b) A serum blank may be used to correct for hemolysis, icterus, or lipemia in patient sera. Suppose one of the patient specimens that you assayed for total protein was icteric. If the absorbance of the serum blank for this patient had an absorbance value of 0.042A, calculate the corrected concentration using the absorbance and concentration data of one of the patients you assayed in the Total Protein assay.

Explanation / Answer

Ans. a. Hemolysis (Red color of serum; due to presence of hemoglobin in serum after RBC lysis), icterus (Yellow color of serum; due to presence of bilirubin) and lipemia (turbidity in serum, due to higher lipid content in serum) increases the absorbance of sample. As a result, these cases may yield a higher serum protein value than the actual one.

Ans. b. Note: The actual absorbance of the sample = Abs of test sample- Abs of serum blank

So, make calculation of the test sample by using its absorbance = (Abs noted- Abs of blank.)

The actual correction factor depends on the method of estimating total proteins in serum, protein concentration of the protein standard, absorbance of the protein standard, absorbance of the test sample, etc.

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