a) Which column has more theoretical plates? (b) which column has a larger plate

Question

a) Which column has more theoretical plates? (b) which column has a larger plate height? ch Which column gives higher resolution? (d) Which column gives a greater relative retention? (e) Which compound has a higher retention factor? (f Which compound has a greater partition coefficient? (g) What is the numerical value of the retention factor of peak A? (h) Wi at is the numerical value of the retention factor of peak B? 1) What is the numerical value of the relative retention? of A and B by column 2 in Problem 23.32. (a 23-33 For th

Explanation / Answer

a) column 1

b)column 1

c)column 1

d)column 1 (slow mobile phase velocity)

e)B(higher peak)

Explanation-

Band broadning is a phenomenon that results from inefficiency of the column leading to poor resolution and chromatographic performance.Van Deemter's equation describes the factors that contributes to band broadning

the equation

HETP=A+B/u +C*u

HETP=height equivalent to theoretical plate(column efficiency)

The factors are-

-A= eddy's diffusion factor, non-ideal packing which makes analyte passing through multiple path in the column

-B=diffusion coefficient a measure of longitudinal molecular diffusion

-C=resistance to mass transfer b/w mobile and stationary phases

u=mobile phase velocity

It is the relation of variance per unit length of column with that of mobile phase velocity.At optimum mobile velocity ,the variance is minimum and hence maximum column efficiency.

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