The enzyme a-chymotrypsin is secreted in the pancreas of mammals and cleaves pep

Question

The enzyme a-chymotrypsin is secreted in the pancreas of mammals and cleaves peptide bonds made between certain amino acids. Several solutions containing the small peptide N-glutary phenylalanine-p-nitroanilide at different concentrations were prepared and the same small amount of a-chymotrypsin was added to each one. The following data were obtained on the initial rates of the formation of product: 2- [Sl/mmol.L10.334 0.450 0.6671.00 1.33 1.67 ro/mmol.Ls 0.152 0.201 0.269 0.417 0.505 0.667 Determine the maximum velocity (k[El) and the Michaelis constant (K) for the reaction

Explanation / Answer

Lineweaver-burk plot is used to determine the KM and Vmax. This is done by

1/V= (KM/Vmax)*1/S + 1/Vmax, so a plot of 1/V vs 1/S is drawn and shown below.

from the plot , intercept 1/Vmax= 0.362, Vmax=1/0.362= 2.76mmol/L.sec

and KM/Vmax= 2.092, KM= 2.092*2.76 mmol/L =5.77 mmol/L

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