4) Which level of protein structure would be most affected by the following chan

Question

4) Which level of protein structure would be most affected by the following changes... (5 pts total) a) mutation from isoleucine to valine b) oxidation of two cysteine sidechains c) PrPe (healthy prion protein, mostly a-helical) changes to PrP (infectious form, mostly B-strand) (more tharn one answer!) d) increase in pH from 7 to 10 5) Decide on the most likely mode of denaturation for each of the following compounds... (4 pts) a) hexane (organic solvent) b) trichloroacetic acid (very strong acid) c) boiling water d) a blender

Explanation / Answer

    Secondary structure because it is changing ? strand to ? strand but because of these changes the overall protein folding that means the tertiary structure will also be changed.

d. Increase in pH will create a change in the charges on protein functional groups this will cause a change in:

5. A) high hydrophobicity in hexane leads to denaturation.

5. B) trichloroacetic acid causes precipitation of proteins by disruption of electrostatic interaction

5. C.) in boiling water Chemical bonds will be broken down ( hydrogen and hydrophobic interactions)

5 d). Due to blender Proteins unfold because of the agitation as their hydrophobic parts get rearranged to avoid water in the favour of air

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