A DNA sequence composed of repeating identical sequences Jack and Jill are under

Question

A DNA sequence composed of repeating identical sequences Jack and Jill are undergoing RFLP analysis. Chromosome 1 has been isolated and digested with restriction enzyme HaeIII. When the results from chromosome 1 were run out on a gel, Jack's DNA produced 13 fragments, while Jill's produced 10. Which of the following describes Jack and Jill's DNA? a. Jack's chromosome has 13 restriction sites, while Jill's has 10 b. Jack's chromosome has 12 restriction sites, while Jill's has 9 c. Jack's chromosome has 14 restriction sites, while Jill's has 11 d. not enough information is provided to answer this question A _____ in a "lawn" of plated bacteria indicates the presence and activity of wild-type bacteriophage that have undergone recombination a. a lytic colony b. lytic phage c. plaque d. lysogenic colony e. none of the above How many complementation groups does the haploid human genome have? 46 23 22 18 None of the above

Explanation / Answer

18)b. Jack has 12 and Jill has 9

Since human DNA is linear, number of restriction sites = n - 1 where n is the number of fragments formed upon restriction digestion by that particular restriction enzyme.

19) e. None of the above

The characteristic of the phage need to be specified to know how its recombinant version would be. Also the site of recombination is also affect it for eg. An insertion at cI gene would make a phage undergo lysis resulting in a plaque while recombination at CRO gene would make a phage undergo lysogeny giving rise to a lysogic colony.

20) b. 23

a complementation group is defined as a group of mutant genes that do not complement each other. In general terms it corresponds to the unique chromosomes of an individual. Hence for a haploid(single set) human genome it is 23.

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