A) A biologist inserted a gene from a human liver cell into the chromosome of a

Question

A) A biologist inserted a gene from a human liver cell into the chromosome of a bacterium. The bacterium then transcribed this gene into mRNA and translated the mRNA into protein. The protein produced was useless; it contained many more amino acids than the protein made by the eukaryotic cell, and the amino acids were in a different sequence. Explain why.

B) Identify and explain the key steps involved in creating a transgenic knock-in mouse.

C) Describe the advantages and disadvantages associated with developing an ex vivo gene therapy approach for treating diseases of the body.

D) Tamiflu® is a commonly prescribed antiviral medication. It works by blocking the viral enzyme, neuraminidase, a protein that helps cleave off the virion during exocytosis. Compare Tamiflu® with a hypothetical antiviral that works instead by blocking coreceptors.

Explanation / Answer

sorry--i put it as a comment: The reason the protein produced in the bacterium was useless was because the gene was inserted (transposed) into the bacterium's chromosome. This means the gene not only its own DNA but it was also surrounded by a foregin organism's. When transcribed into mRNA to be translated to a protein, the coding can completly change the codons surrounding the inserted gene and there is a chance that a stop codon can be translated before it reaches the human liver gene, or it could (as in this case) be translated just with the surrounding bacterium chromosome and therefore give it an entire different coding for the protein. I know i just answered A but i hope it helped!

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