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Select the correct characteristics describing normal-phase and reversed-phase ch

ID: 996007 • Letter: S

Question

Select the correct characteristics describing normal-phase and reversed-phase chromatography.

Polarity of phases: Stationary phase is polar. Stationary phase is non-polar. (b) Eluent strength of Solvent: Increases as Solvent becomes more polar. Increases as Solvent becomes less polar. (c) Nature of solutes: Polar Non-polar (d) Nature of solute interaction: More soluble in mobile phase as polarity increases. More soluble in mobile phase as polarity decreases. (e) Polarity of phases: Stationary phase is polar. Stationary phase is non-polar. (f) Eluent strength of Solvent: Increases as Solvent becomes more polar. Increases as Solvent becomes less polar. (g) Nature of solutes: Polar Non-polar (h) Nature of solute interaction: More soluble in mobile phase as polarity increases. More soluble in mobile phase as polarity decreases.

Explanation / Answer

Nomal phase chromatography

(a) Polarity of phases :

Stationary phase is polar

(b) Eluent strength of solvent :

Increases as solvent becomes more polar

(c) Nature of solutes :

Non-Polar

(d) Nature of solute interaction :

More soluble in mobile phase as polarity increases

Reverse phase chromatography

(e) Polarity of phases :

Stationary phase is non-polar

(f) Eluent strength of solvent :

Increases as solvent becomes less polar

(g) Nature of solutes :

Non-Polar

(h) Nature of solute interaction :

More soluble in mobile phase as polarity decreases

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