Select the best method for isolating a mesophilic, Gram-negative enteric capable

Question

Select the best method for isolating a mesophilic, Gram-negative enteric capable of fermenting lactose.

Perform isolation streak onto MSA, grow at 37C, select colonies that yellowing around them.

Perform isolation streak onto MAC, grow at 37C, select pink colonies.

Perform isolation streak onto BAP, grow at 37C, select colonies with clearing around them

Perform isolation streak onto MAC, grow at 15C, select pink colonies.

Perform isolation streak onto MSA, grow at 37C, select colonies that yellowing around them.

Perform isolation streak onto MAC, grow at 37C, select pink colonies.

Perform isolation streak onto BAP, grow at 37C, select colonies with clearing around them

Perform isolation streak onto MAC, grow at 15C, select pink colonies.

Explanation / Answer

Mesophilic bacteria grow best between 20 and 45 c.
They are allowed to grow on MAC agar and perform isolation streak.
Once they are grown, the pinkish colonies are selected.
Option b is the correct answer.

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