Enzyme E catalyzes the conversion of substrate A to product B. The Lineweaver-Bu
ID: 99088 • Letter: E
Question
Enzyme E catalyzes the conversion of substrate A to product B. The Lineweaver-Burk plot depicting this conversion is shown as a solid line. The kinetics after addition of compound X to the reaction mixture are also plotted as a dashed line. The kinetics of a mutant version of E called E* are also depicted on the plot. You measure the kinetics for the reaction with E* in the presence of X and find that they are identical to the kinetics of E*without X (data not shown). Based on all these data, answer the following questions: a) What is the MOST LIKELY conclusion about the effect of X on E? Explain. b) What conclusion can you make about the effect of the mutation on E? Explain. c) What is the BEST explanation for the effect of X on E*? Explain.Explanation / Answer
a) The compound X acts as a competitive inhibitor to E. This is because, Vmax remains unaltered but Km has increased.
b) The mutation on E has resulted in the increased rate of reaction.
c) The compound X acts as a uncompetitive inhibitor of E*. This is because both Vmax and Km is reduced.
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