Enzyme E catalyzes the conversion of substrate A to product B. The Lineweaver-Bu
ID: 98941 • Letter: E
Question
Enzyme E catalyzes the conversion of substrate A to product B. The Lineweaver-Burk plot depicting this conversion is shown as a solid line. The kinetics after addition of compound X to the reaction mixture are also plotted as a dashed line. The kinetics of a mutant version of E called E* are also depicted on the plot. You measure the kinetics for the reaction with E* in the presence of X and find that they are identical to the kinetics of E* without X (data not shown). Based on all these data, answer the following questions: a) What is the MOST LIKELY conclusion about the effect of X on E? Explain. b) What conclusion can you make about the effect of the mutation on E? Explain. c) What is the BEST explanation for the effect of X on E*? Explain.Explanation / Answer
15a)The enzyme E is involved in the conversion fo substrate A to B ,after which a compund X is added for which the lineweaver-burk plot is shown in the above graph of 1/[S] and 1/vo, so the effect of X on E is competitive inhibition.
b)The mutaion in enzyme E leading to the formation of enzyme E* explains that the compound X has no effect on the inhibition kinetics of the mutant enzyme.
c)After the converion of enzyme E to mutant form E*, a compound X is added but the lineweaver-Burk plot kinetics is not changed after the addition of compound X for the mutant enzyme E* which explains that the compound X has no effect as the catalytic active site of the mutant enzyme does not have any effect on the compound inhibitor X kinetics with mutant enzyme E*.
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