Protein purification. The sequences of four polypeptides are written below (usin

Question

Protein purification. The sequences of four polypeptides are written below (using single-letter amino acid codes). Polypeptide #1: ACDEFGHI Polypeptide #3: HHHHHH Polypeptide #2: GGG Polypeptide #4: KLMNPQRVWY Identify which polypeptide would migrate slowest during chromatography. [In the spaces provided, write the number of the polypeptide that migrates slowest.] A) Chromatography resin: diethylaminoethyl (DEAE)- cellulose (at pH 7.4 in 100 mM NaCl) Slowest moving polypeptide [Write #1, #2, #3, or #4.] _ B) Chromatography resin: carboxymethyl (CM)-cellulose (at pH 7.0 in 50 mM NaCl) Slowest moving polypeptide [ Write #1, #2, #3, or #4.] _ C) Chromatography resin: gel filtration (at pH 7.0 without NaCl) Slowest moving polypeptide [Write #1, #2, #3, or #4.] _ D) Chromatography resin: Ni2+ -NTA agarose (at pH 7.4 in 100 mM NaCl) Slowest moving polypeptide [Write #1, #2, #3, or #4.) _

Explanation / Answer

a) #2 - (1)

b) #4 -(6)

c) #4

d) #3

all are calculated using pH values

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