Second, you will make 10ml of 100 mu g/ml. How much 2000 mu g/ml BSA will you al

Question

Second, you will make 10ml of 100 mu g/ml. How much 2000 mu g/ml BSA will you all to make 10 mL of 100 mu g/ml? Next, you will make 1 ml of 5 mu g/ml. Add mu l of your 100 mu g / ml BSA and mu l of water (to make 1ml total). Next, you will make 1ml of 10 mu g/ml. Add mu l of your 100 mu g/ml BSA and mu l of water (to make 1ml total) Next, you will make 1 ml of 20 mu g/ml. Add mu l of your 100 mu g/ ml BSA and mu l of water (to make 1 ml total). Add mu l of your 100 mu g/ml BSA and mu l of water (to make 1 ml total) Nesxt, you will make 1 ml of 30 mu g/ml. Add mu l of your 100 mu g/ml BSA and mu l of water (to make 1 ml total).

Explanation / Answer

4a) The solution we will make is 2mg/mL, this means that there are 2mg per 1mL, so if we need to prepare 10mL we will need 2mg x 10:

1 mL ------ 2mg

10 mL ---- x= 20mg ---> 0.02g

4b) We need to prepare 10 mL of 100ug/mL, this solution contains 100ug in 1mL, so, in 10mL we will have 1000ug.

We have to take 1000ug from the 4a solution:

2000ug ------- 1mL

1000ug ------- x= 0.5mL ----> so take o,5mL of 4a and add water until 10mL.

Another way to see this (easier way) is doing this:

Cstock x Vstock= Cdiluted x Vdiluted

In this case we want to know the volume of the stock solution needed to prepare the siluted one:

Vstock= 100ug/mL x 10mL/2000ug/mL ---> Vstock =0.5mL

Do it the way you want

4c) Cs xVs= Cd x Vd

Vs= 5ug/mL x 1mL/100ug/mL= 0.05mL + 0.95mL of water

4d) Cs xVs= Cd x Vd

Vs= 10ug/mL x 1mL/100ug/mL= 0.1mL + 0.9mL of water

4e) Cs xVs= Cd x Vd

Vs= 20ug/mL x 1mL/100ug/mL= 0.2mL + 0.8mL of water

4f?) Cs xVs= Cd x Vd

Vs= 30ug/mL x 1mL/100ug/mL= 0.3mL + 0.7mL of water

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