The pAMP plasmid below has several restriction sites, as shown in the diagram be
ID: 9868 • Letter: T
Question
The pAMP plasmid below has several restriction sites, as shown in the diagram below. The total length is 4539 base pairs. Location of restriction sites are noted by the numbers below each restriction site (for example, SacII cleaves at a reference point of 380 base pairs from the EcoRI site).
Several different experiments are conducted in which the pAMP plasmid is digested with different combinations of restriction enzymes (A through D below). For each of the cases A through D, calculate the number of fragments and each fragments theoretical size.
A-NarI only
B-SacII and HindIII
C-BamHI, EcoRI, and MluI
D-All enzymes
Explanation / Answer
A-NarI only
The total length is 4539 base pairs
2 fragments --------171 &,4368 base pairs
=======================
B-SacII and HindIII
3 fragments
380 base pairs ,1524 base pairs,2635 base pairs
============================
C-BamHI, EcoRI, and MluI
3 fragments
994 base pairs, 204 base pairs ,3419204 base pairs
===============
D-All enzymes
8 fragments
380 base pairs,614 base pairs,126 base pairs,79 base pairs,705 base pairs,2464 base pairs,235 base pairs,171 base pairs,
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