Learn how to extract and separate plant pigments using thin layer chromatography
ID: 981175 • Letter: L
Question
Learn how to extract and separate plant pigments using thin layer chromatography. Pre-Lab Assignment: Take the prelab quiz online. Some of the following questions are part of the online quiz though the format of the question might change. Prepare your notebook according to guidelines. Cut and tape the procedure into your lab notebook. As you are working with your lab partners in lab answer questions embedded in the procedure. Prelab questions. (PLI) What docs TLC stand for (PL2) Define TLC, mobile phase, and stationary phase. What is the mobile phase for this lab The stationary phase (PL3) If the organic compound has more C's and H's (larger hydrocarbon), it is considered more or less polar than the compound that has less C's H's(smaller hydrocarbon) Briefly explain. (PL4) If the organic compound has more electronegative elements like O's. N's and halogens, it is considered more or less polar than the compound that has less number of electronegative elements Briefly explain. (PL5) Using the logic from PL 3 and PL4 and formulas given here, put these three pigments in increasing polarity. Chlorophyll a (C_55H_72MgN_4O_5), Chlorophyll b (C_55 H_70MgN_4O_6), beta-Carotene (C_40H_56)Explanation / Answer
(1) TLC stand for Thin Layer CHromatography.
(2) T his technique is used to separate volatile compounds which is performed on glass, plastic or aluminium foil which is coated with a thin layer of adsorbent.
The thin layer of adsorbent like silica gel, aluminium oxide or celluose is know as stationary phase.
After sample has been applied on the plate, a solvent is drawn up the plate via capillary action, different analytes ascend the TLC plate at different rates. This solvent is kanown mobile phase.
(3) Compounds having more C's and H's are less polar compared to compounds having less C's and H's .
(4) COmpounds containing O's and N's are more polar than compounds containing C's and H's due to difference in electronegativities of C and O or N.
(5) Beta Carotene < Chlorophyll a < Chlorophyll b
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