Figure 6-30 p168 4. Table 3.1 of your G&G text gives some thermodynamic values f

Question

Figure 6-30 p168

4. Table 3.1 of your G&G text gives some thermodynamic values for the denaturation of several proteins, i.e., unfolding of the polypeptides, under various conditions, e.g. moderately high or low pH. Among the proteins are myoglobin and RNase. Later in your text, Fig. 6.30 shows the fractional unfolding of RNase A and B as a function of temperature.

a. Use the temperatures in Fig. 6.30 at which RNase A is 25, 50, and 75% unfolded to make a van’t Hoff plot. Use Excel to tabulate your data and make the plot. Include a trendline and its equation.

b. From the plot determine H´ and S´ for the denaturation reaction. What are the corresponding values for RNase A folding?

c. What is G°´ for RNase A folding at 30? In making this calculation, assume H´ and S´ are constant with respect to temperature.

d. Is the RNase folding driven by entropy or enthalpy? State the basis for your answer.

e. How do the thermodynamic values you calculated compare to those for myoglobin and RNase in Table 3.1? What could explain any differences?

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