using the table above. For paper electrophoresis, predict if each of the followi
ID: 979757 • Letter: U
Question
using the table above.
For paper electrophoresis, predict if each of the following amino acids would migrate rapidly towards the cathode (negative pole), remain approximately stationary, or migrate rapidly towards the anode (positive pole) at each of the following pH values: pH 3, 5.5, and 10.
a. Aspartic Acid b. Arginine c. Threonine
Table 3.2 pKa values of acidic and basic constituents of free amino acids at 25°C Amino acid pKa value Carboxyl Amino Side group group chain 9.8 9.9 9.7 9.7 9.8 Methionine 2.1 9.3 10.6 9.3 Tryptophan 2.5 9.4 9.2 9.1 2.4 2.4 2.3 2.3 2.3 Glycine Alanine Leucine Isoleucine Proline 2.0 Phenylalanine 2.2 Serine Threonine 2.1 Cysteine 1.9 10.7 8.4 Tyrosine 2.2 9.2 10.5 Asparagine 2.1 Glutamine 2.2 Aspartic acid 2.0 9.9 3.9 Glutamic acid 2.1 Lysine Arginine 1.8 Histidine 2.2 3 8.7 9.5 4.1 2.2 9.1 10.5 9.0 12. 9.3 6.0Explanation / Answer
For paper electrophoresis, the prediction of amino acid movement,
a. aspartic acid
at pH 3.0 : migrate towards cathode
at pH 5.5 : migrate towards anode
at pH 10 : migrate towards anode
b. Arginine
at pH 3.0 : migrate towards cathode
at pH 5.5 : migrate towards cathode
at pH 10 : remain approximately stationary
c. Threonine
at pH 3.0 : migrate towards cathode
at pH 5.5 : migrate towards cathode
at pH 10 : migrate towards anode
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