Date Chapter 5.qsiz Class: Indicate the answer choice that best completes the st

Question

Date Chapter 5.qsiz Class: Indicate the answer choice that best completes the stateme -1. Which separates on the basis ofmolecular weight? at best completes the statement or answers the question. a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange -2. Which would be best to separate positively charged proteins? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange 3. The isoelectric point is a. the pH at which a substance has no net charge b. the pH at which a substance has a net positive charge c. the pH at which a substance has a net negative charge d. the pH at which a substance has no charge groups of any kind 4. Which would be best to separate a protein that binds strongly to its substrate? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange -5. Studying how proteins interact with other proteins is called a. expression proteomics b. structural proteomics c. interaction proteomics d. none of the choices

Explanation / Answer

1. A) gel filtration chromatography

In gel filtration chromatography the particles are separated on the basis of size, with the largest sized molecules eluting first and the smallest size molecules luring in the end as they get trapped in the pores of the beads of the gel. This chromatography is also called gel permeation chromatography or molecular exclusion chromatography.

2. C) cation exchange

In cation exchange chromatography, an ion exchange resin that is negative in charge is used to separate the molecules that would have a surface charge which is positive.

3. A) The pH at which a substance has no no net charge

The isoelectric point of a molecule is the one in which the molecule would not carry any net charge. The isoelectric point is commonly denoted by pI.

4. B) Affinity chromatography

Affinity chromatography is the chromatographic technique in which the molecules are separated on the basis of a ligand and a receptor or an antigen or an antibody.

5. C) interaction proteomics

This is the branch of proteomics that is involved in studying protein interactions. This is done by the study of the interacting proteins and their network, in order to understand the cellular interactions.

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