pling In gas chromatography, what are the advantages of (a) temperature programm
ID: 966344 • Letter: P
Question
pling In gas chromatography, what are the advantages of (a) temperature programming? (check all that apply.) V Resolution of low-boiling solutes is maintained Retent on times of high-boiling solutes are decreased The elution order is reversed: high-boiling solutes are eluted prior to low-boiling solutes Sample capacity increases. (b) pressure programming? (check all that a O Resolution of low-boiling solutes is maintained O Retention times of high-boiling solutes are decreased Thermal degradation of samples is less likely. It isn't necessary to wait for a hot column to cool before injecting another sample.Explanation / Answer
For thermal programming:
In gas chromatography if you run a "isothermal" experiment you will find that the first peaks will be well defined but the experiment will take too much so you program the temperature in order to reduce the time the other components need to elude, you start with low temperatures and then you increase it gradually
Resolution of low-boiling solutes is maintained
Retention times of high-boiling solutes are decreased.
For pressure programming: (All four are correct)
When you program the pressure you will run the experiment in a range of pressures, when you increase the pressure the flow also increases this will reduce the retention time.
At the end of the run you can reduce the pressure, this will save you time from waiting for the column to cool down, this also helps the analyte to avoid degradation because of high temperatures
-Resolution of low-boiling solutes is maintained.
-Retention times of high-boiling solutes are decreased.
-Thermal degradation of samples is less likely.
-It isn 't necessary to wait for a hot column to cool before injecting another sample.
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