Which of the following processes accounts for the tremendous variation in antibo

Question

Which of the following processes accounts for the tremendous variation in antibody structure? A) KNA editing B) Size-specific recombination C) integrate activity D) Physiological adaptation E) Hot spots of mutation in the immunoglobin genes RT-PCR is used for which of the following A) Conduct a PCR reaction without the use of primers B) Generate cDNA C) Quantity KNA in a cell D) Nonspecific DNA amplification E) None of the answers are correct are used in which of the following techniques? A) RT-PCR B) DNA sequencing C) DNAse I foot printing D) Restriction mapping E) Gene cloning Which enzyme is unique to the retro elements? A) Reverse transcriptase B) Reverse resolvase C) Transposase D) Integrase E) Reverse integrase DNA usage is needed in a cloning experiment for what reason? A) To proofread the vector for mistakes B) To reestablish the stable sugar-phosphate backbone of the DNA molecule C) To allow the vector to enter into the cell D) As a selectab

Explanation / Answer

Answer:

10. (B) Site specific recombination

Explanation:

Large variations in antibody structure is brought about by site specific recombination which occurs between specific DNA sequences, and are homologous over a short stretch of DNA.

11. (B) Generate cDNA

Explanation:

Reverse transcriptase PCR exploits the ability of reverse transcriptase to synthesize cDNA from mRNA and use it as a template to amplify target sequences.

12. (B) DNA sequencing

Explanation:

Dideoxynucleotides are chain terminating nucleotides that are used to sequence DNA through Sanger sequencing.

13. (A) Reverse transcriptase

Explanation:

Retroelements are mobile genetic elements that transpose via an RNA intermediate and are reverse transcribed to DNA by reverse transcriptase which integrate into a new location.

(Since there are multiple questions, the first 4 questions have been answered according to the rules of chegg)

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