You have isolated a temperature-sensitive mutant that, at the restrictive temper

Question

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type.

What is your hypothesis for a protein that may be responsible for this mutant phenotype.

Explanation / Answer

All the transporter proteins are normal so it means that all the translation level things are correct so it might be possible that Carrier which is carrying the protein into mitochondria has mutated TRANSCRIPTION FACTOR responsible for making mrna for the carrier protein and as the mrna is mutated, carrier protein is not made and the proteins are not able to translocate in the mitochondria.

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