You have isolated a temperature-sensitive mutant that, at the restrictive temper

Question

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type. What is your hypothesis for a protein that may be responsible for this mutant phenotype.

Explanation / Answer

For a protein to get translocated into the cell , first it should have a signal sequence . This signal sequence has a specific signal which directs the protein to mitochondria. If this signal sequence is lost then the protein cannot be translocated to the mitochondria. So here the proteins are not translocated into the mitochondria but the translocators have no mutation. Hence there might be a mutation in the protein processing step. So the protein is not translocated into mitochondria. So there might be a mutation in transcription or translation step.    Hence the product has a mutant phenotype.

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