1.A given DNA molecule, when digested with either HpaII or MspI, gives three and

Question

1.A given DNA molecule, when digested with either HpaII or MspI, gives three and five
fragments, respectively. Given that these two enzymes recognize the same sequence, how can
you account for the different numbers of fragments produced?

2.Melissa Moore and Phil Sharp devised a technique to create a pre-mRNA that contains a
single deoxyribonucleotide at a specific position. You have used this technique to make three
pre-mRNAs, each of which is composed entirely of ribonucleotides except for a single
deoxyribonucleotide at one specific site. The first pre-mRNA has a deoxyribonucleotide at the
first nucleotide of the intron, the second has its deoxyribonucleotide at the branch site, and the
third has it at the last nucleotide of the intron. Do you expect these three pre-mRNAs to be
spliced?

Explanation / Answer

C!CCG GGC!C

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