1 Which of the following is a property of the detergent SDS? A. Disrupt three di

Question

1 Which of the following is a property of the detergent SDS?

A. Disrupt three dimensional structure (denature) of proteins

B. Bind to protein and increase negative charge associated with it

C. Break disulfide linkages

D. Catalyze polymerization of acrylamide

E. Both A and B

2 You should wear gloves when doing which of the following?

A Handling a polymerized polyacrylamide gel

Pouring your own polyacrylamide gel starting with liquid acrylamide

Handling an agarose gel containing SYBR Safe dye

All of the above

A Handling a polymerized polyacrylamide gel

Pouring your own polyacrylamide gel starting with liquid acrylamide

Handling an agarose gel containing SYBR Safe dye

All of the above

Explanation / Answer

1.

A)Sodium dodecy sulfate synonymsly sodium lauryl sulfate (SDS orSLS) compound with the formula CCH3(CH2)11SO4Na.it is an anionic surfactant used in many cleaning and hygiene products.it consists of a 12-carbon tail attached to a sulfate group,it is the sodium salt of dodecy hydrogen sulfate the ester of ddecyl alcohol and sulfate acid.

B)as crystallization of membrane proteins, which generally involves the careful selection of solubilizing detergent and mixed lipid/detergent systems.

So answer is E both A and B

2.Sybr safe is a cyanine dye used as a nucleic stain in molecular biology.

Sybr safe is a safer alternative to the known mutagen ethidium bromide although it stills carcinogen,as it binds DNA just like ethidium bromide as with all forms of gels electrophoresis, molecules may be run in their native sate.preserving the molecules higher order structure.this method is called native page.

So we wear gloves in the all above situations.when this activity's done..

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