1. A process whereby \"naked\"/cell-free DNA enters a recipient cell is called a
ID: 95247 • Letter: 1
Question
1. A process whereby "naked"/cell-free DNA enters a recipient cell is called a. transduction b. transformation c. conjugation 2. A cell that allows cell-free DNA to enter by passing through its cell wall and cell membrane has to be a.) competent b. mutated c. lysed 3. Which characteristic(s) were required by the Escherichia coli strain used for the transformation experiment? inability to catabolize lactose both a andb b. susceptibility to penicillin 4. The plasmid used for the transformation was c. pBR a. pBAD Blu 5· The plasmid used in the transformation exercise had a gene for penicillin resistance and the a. tryptophan operon (b.actose c. ampicillin 6· The medium on which the transformation occurred was Luria-Bertani agar containing as a selective ingredient and as a differential ingredient a) ampicillin; X-Gal b. X-Gal; ampicillin c. ampicillin; lactose Act like scissors in recognizing and cutting DNA in particular sequences called palindromes a. ligases 7. b.restriction endonucleases c. exonucleases 8. The following is an example of a palindrome During gel electrophoresis DNA fragments migrate from the a. negative; positive a.) faster than larger, longer DNAs 9. pole to the pole based on size b. positive; negative c. none of these 10. Shorter, smaller DNAs move b. slower than larger, longer DNAs c. at the same rate as larger, longer DNAS circular DNA migrates to the pole faster than circular DNA DNA closed: positive; nicked; linear b. linear: positive; closed; nicked b. nicked: positive; closed; linearExplanation / Answer
ANSWER:
1) Transformation
2) Competent
3) Both a and b
4) pBlu
5) Lactose
6) ampicillin;X-Gal
7) Restriction endonucleases
8) 5'AATTC3'/3'CTTAA5'
9) Negative;Positive
10) Faster than larger,Longer DNAs
11) closed;positive;nicked;linear
12) phenotype
13) missense and point mutations
14) frame shift
15) b
16) a
17) d
18) point mutation
19) ultra violet radiation
20) transduction
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.