1. A polylinker (also known as a multiple cloning site) is a: . 2. Generating a
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Question
1. A polylinker (also known as a multiple cloning site) is a:
.
2. Generating a recombiant DNA vector in vitro with a fragment of chromosomal DNA requires __________.
a) DNA polymerase
b) DNA ligase
c) restriction enzymes
d) B and C
e) A,B,C
.
3.
A) synthetic DNA fragment that contains multiple restriction enzyme sites B) synthetic DNA fragment inserted into a vector that allows replication of the vector in the host cell C DNAequence that allows for selection of cells that contain the vector D) molecule used to ligate DNA fragments together E) synthetic DNA fragment used to screen or visualize colonies that contain the DNA of interestExplanation / Answer
Q.1
Option - A
It is a synthetic DNA fragment which has multiple cloning site or otherwise known as restriction enzyme site.
Q.2
Option - D.
To generate the recombinant DNA vector, the chromosomal DNA and the vector has to be digested with the restriction enzyme and then sealed using DNA ligase.
Q.3
Option B.
The blue color colonies represent the empty vector, which means that the functioning beta galactosidase is intact.
Whereas white colonies represent the vector with DNA insert.
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