Why are dATP, dCTP, dTTP and dCTP added to a PCR reaction tube? They catalyze th

Question

Why are dATP, dCTP, dTTP and dCTP added to a PCR reaction tube? They catalyze the polymerase. They provide the building blocks of DNA. They buffer the mixture. They allow the DNA in the sample to anneal. Why are universal 16S rDNA primers used in your experiment? They will anneal to highly conserved areas of the gene that encodes bacterial 16S rRNA. They will anneal to unique sequences of genes encoding 16S rRNA in specific bacteria. If universal primers are used to amplify DNA in a PCR reaction, then the PCR product must be sequenced to determine the. True false How is the PCR product separated from the PCR mixture at the completion of the reaction? Perform electrophoresis in an agarose gel, stain the gel and cut the band corresponding to the PCR product from the gel Pour the PCR mixture into a commercially prepared DNA microconcentrator column and follow the manufacturer's direct product from the column. Both of the above procedures may be used. Neither of the above procedures may be used.

Explanation / Answer

Answer:

14. (A) They will anneal to highly conserved areas of the gene that encodes bacterial 16S rRNA.

Explanation:

16s rRNA sequences consists of conserved and variable regions. This highly conserved rRNA sequences makes it possible to PCR amplify rRNA sequences from a wide range of bacteria through the design and use of universal primers.

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