https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0130-0 This is the l

Question

https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0130-0

This is the link to the research article

1. Describe in 3 sentences or less WHY the authors conducted this research – what was their goal? (4pts)

2. For each of the figures (1-5) provide a one sentence description of the PURPOSE of the figure AND a one sentence CONCLUSION of the experiments in the figure. (15pts) Note that: a. You only need to provide one sentence for the entire figure, not for each panel (a, b, etc.) of the figure. This requires you to summarize info from multiple panels into a concise and informative sentence. b. The purpose of the experiment is a ‘Why’ not a ‘how’ or ‘what’. Your answer should not describe what the figure shows, but why the experiments were done, i.e, what was the goal of doing the relevant group of experiments. c. The conclusion is the take-home message of the figure. As immediately above, your answer should not be a description of what the figure shows, but what the researchers deduce from the experiment

3. In your view, what is the most important figure in the paper? Why? (3pts)

4. In your view, what is the next logical experiment that should be performed given the results presented in the paper? (3pts)

Explanation / Answer

Glioblastoma is the common form of brain cancer. The major cause for this is an aberrant glucose metabolism and here two metabolism targeting drugs PENAO and DCA effect on the glioblastoma and their synergistic mechanisms is discussed.

Fig 1 purpose is that to show cell viability or growth in presence of DCA, PENAO and combined. the different ways of checking the viability shows the combined effect on cells have a low survival rate compared to individual and compared to control.

Fig 2 shows the cytotoxicity effects of the compounds individually and in combination and shows that the DCA enhances the cytotoxicity of PENAO

Fig 3 regulation of PENAO-DCA cytotoxicity by the redox system, findings indicate GSH-regulated redox change plays a pivotal role in the cytotoxic effect of PENAO-DCA treatment.

Fig 4 western blot successfully demonstrated that the expression of MRP1 in both U87 and BAH1 cells was down-regulated after 24 hr DCA treatment

Fig 5  anti-metabolic effect of PENAO-DCA combination, two metabolic parameters, oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), were measured and the DCA is promoting the oxidative phosphorylation and decreases acid production by PENAO.

3. The most important figure is figure 4 and the expression of MRP 1 protein effect of the compounds on the MRP1 resistance to treatment is very interesting here.

4. in Vivo studies on the combination of compounds in the treatment of glioblastoma.

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