You have an oligonucleotide probe that hybridizes to part of gene A from a eukar

Question

You have an oligonucleotide probe that hybridizes to part of gene A from a eukaryotic cell. In order to carry out your work you need to make a DNA library. You have the choice of making either a genomic DNA library or a cDNA(copy DNA) library made from mRNA isolated from the cell that has been reverse transcribed back to DNA. Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications. Explain your answers. You discover that gene A is alternatively spliced and you want to see which predicted alternative splice products the cell actually produces. You want to express gene A in bacteria to produce lots of protein A.

Explanation / Answer

Ans. A) In the given situation, where Gene A is alternatively spliced and one has to observe which predicted alternative splice products are actually produced in a cell. It is required to express the entire gene to check their products. Hence, preparation of genomic library would give better results.
(The genomic library have all the DNA fragments that signify the complete genome of an organism, on the other hand cDNA library is constructed with particular mRNA of specific cell from an organism.)

B.) In the given situation, where it has to be determined that gene A in bacteria must produces lots of protein A. cDNA library would be better option. By the application of cDNA library, one can produced as much as desired product or protein.

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