You have an oligonucleotide probe that hybridizes to part of gene A from a eucar

Question

You have an oligonucleotide probe that hybridizes to part of gene A from a eucaryotic cell. You can use this probe to isolate a corresponding plasmid from a DNA library harbored by a collection of bacteria. Will a cDNA library or a Genomic DNA library be more appropriate for the following applications? Explain

A. You want to study the promoter of a gene A.
Type of library?_____________ Explain.

B. Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of of the tRNA
Type of library?_____________ Explain.

C. You discover that gene A is alternatively spliced and you want to see which predicted alternative splice products are actually produced in a cell.
Type of library?_____________ Explain.

D. You want to find both Gene A and the genes located near Gene A on the chromosome.
Type of library?_____________ Explain.

E. You want to express Gene A in bacteria to produce lots of protein A.
Type of library?_____________ Explain.

Explanation / Answer

Hi there, A. You want to study the promoter of a gene A. Type of library?DNA library.Because promoter sequence is not availabe in cDNA library.It is present before the gene in DNA library. B. Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of of the tRNA Type of library? cDNA library.Because tRNA binds to mRNA during translation.So the cDNA which is the complimentary of the mRNA should help in knowing the gene sequence of t RNA. C. You discover that gene A is alternatively spliced and you want to see which predicted alternative splice products are actually produced in a cell. Type of library? Both DNA and cDNA library.Because spliced products need to be identified by cDNA and compare with DNA library for which part is not involved in cDNA.That is spliced off. D. You want to find both Gene A and the genes located near Gene A on the chromosome. Type of library? DNA library.cDNA libary refers for mRNAs coded for one gene.So the other gene information is present only at DNA level. E. You want to express Gene A in bacteria to produce lots of protein A. Type of library? cDNA library.Because the DNA could be synthesized from cDNA and tranformation could be done and the product can be expressed from that.

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