Draw the product of transcription. The following sequence contains two small gen
ID: 93553 • Letter: D
Question
Draw the product of transcription.The following sequence contains two small genes in E. coli The -10 hexamers of both promoters are boxed. the product of transcription in the space below the diagram for the transcript from the stronger of the two promoters. (note: inverted repeats are signified by arrows over the complementary sequence). For the purposes of this question, transcription termination in a p-dependent terminator occurs 7 bases beyond the end of the stem loop. A p-independent terminator terminates as described in class. Be sure to label the 5' and 3' ends of your RNA. Draw your RNA with the stem structure of the terminator (draw lines to represent H-bonds between the complementary base pairs in the stem loop) ITTATTCAA ACTTGAAA ATAAGO GANG AGT GGTTTTTT ICCA GAAGGCATAATAT TCArrfr ATTATA CAGCTGTATAAGAAA. CTTCCGTATTATAAGTAaB. Potential nut site CAAG ATGGCACTcGCATCG TTGAGGcGCTTGTT GTGTGATCCT AATAT TTTTAGTCAGAGTTOGTAccGTGAG CGTAGC TCACA CGITEGAO ATTAAA TTTTTTAAGCATCGAGCGA C-3 AGTG GCAAACTGTA ATT TGCAC GTAG CTCGCTG-5 Potential rut site Draw the product of translation of your mRNA in the space below. The minimal shine-Delgarno consensus sequence is 5'-AGGAGG-3'. Label N- and C-termini. Show a single base pair substitution on the above sequence that will increase the size of the transcript. Describe an intragenic suppressor of the mutation you showed above.
Explanation / Answer
Given DNA
ggtttttttccacgctctagtgctagagcgtggcataagcgaagtcaagtttattcaattaaacttgaaagaaggcataatattcatttatattaggccctcctcagcctgaggaggattatatgcagctgtataagaaaaatataattgaggcgcttgttgtgtgatcctgttgagactgaaaaatcagtctcaagcatggactcgcatcgtcacacgtttgacattaaacgtgtgtttttttaagcatcgagcgac
(1) The protein from reading frame 2 - GTTTTTTTCCACGCTCTAGTGCTAGAGCGTGGCATAAGCGAAGTCAAGTTTATTCAATTAAACTTGAAAGAAGGCATAATATTCATTTATATTAGGCCCTCCTCAGCCTGA has highest amino acid length - VFFHALVLERGISEVKFIQLNLKEGIIFIYIRPSSA (36 amino acids).
(2) mutated DNA gives a protein VFFHALVLERGISEVKFIQLNLKEGIIFIYIRPSSACRRIICSCIRKI - length 48 amino acids
ggtttttttccacgctctagtgctagagcgtggcataagcgaagtcaagtttattcaattaaacttgaaagaaggcataatattcatttatattaggccctcctcagcctg(T)ggaggattatatgcagctgtataagaaaaatat
(3) pseudo reversion of TGT to TGA might be an intragenic suppressor for the mutation.
(4) VFFHALVLERGISEVKFIQLNLKEGIIFIYIRPSSACRRIICSCIRKI - length 48 amino acids
ggtttttttccacgctctagtgctagagcgtggcataagcgaagtcaagtttattcaattaaacttgaaagaaggcataatattcatttatattaggccctcctcagcctg(T)ggaggattatatgcagctgtataagaaaaatat. This would not affect the transcript produced
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