In Liquid Chromatographic Analysis of Analgesic Powders Lab Prepare three mixed

Question

In Liquid Chromatographic Analysis of Analgesic Powders Lab

Prepare three mixed standard solutions using your 10 mL volumetric flask, transferring each solution to a vial. Prepare the mixed standards by pipette o.33 mL of each stock solution into the 10 mL flask and dilute with the mobile phase.

Obtain an unknown analgesic powder. Accurately weigh out 100 mg sample and dissolve it in 100 mL of 50% mobile phase and 50% water using 100 volumetric flask. Then dilute the solution to the mark using distilled water. Further dilute the sample solution 1:10 in the mobile phase using a pipette and your 10 mL volumetric flask. Transfer the sample solution to a vial.

the result from HPLC

[1] The standard

[2] The unknown sample

Q: From the standard and the sample graph, Identify the peaks in each of the chromatograms.

The compound are
[1] 600 ppm acetaminophen
[2] 1200 ppm acetyl salicylic acid
[3] 100 ppm caffeine

# Time Area Height Width Area% Symmetry 1 0.839 771.7 254.8 0.0473 80.889 0.771 2 1.041 62.7 18.1 0.0525 6.569 0.775 3 1.27 119.6 34.1 0.0539 12.542 0.757 PO ED TDO 0 0 02 04 06 08 12 16

Explanation / Answer

acetaminophen will have least retention time and hence the highest peak. Hence the first peak having the highest highest and area will be acetaminophen.

acetyl salicylic acid will have highest retention time. Hence the third peak will be acetyl salicylic acid.

The second peak having intermediate retention time will be of caffeine.

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