The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal t

Question

The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal translocation (movement of the ribosome relative to the mRNA). It does not affect any other step in protein synthesis. Suppose you are doing a translation reaction in vitro and you add erythromycin just before the 3rd peptide bond is about to be made. You wait a few minutes and look at the ribosome.

Circle the correct answer:

a. The carboxyl end of AA #3 should now be covalently bonded directly to: (tRNA #2) (tRNA #3) (tRNA #4) (AA #2) (AA #3) (AA #4) (none of these).

b. Which tRNA will you find in the A site? (tRNA #1) (tRNA #2) (tRNA #3) (tRNA #4) (tRNA #5).

c. The tRNA in the A site will carry: (an amino acid) (a polypeptide) (neither) (either one)

Explanation / Answer

a. The carboxyl end of AA #3 should now be covalently bonded directly to AA # 4 because erythromycin do not inhibit the process of peptide bond formation, it inhibits the translocation of mRNA along ribosome

b. In the A site, we will find tRNA # 3

c. Always tRNA in the A site will carry amino acid only, the tRNA at P site only carry the polypeptide.

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